Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary.
Identifieur interne : 000E25 ( France/Analysis ); précédent : 000E24; suivant : 000E26Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary.
Auteurs : Jérôme Cachot [France] ; Florence Bultelle ; Laurent Drouot ; François Galgani ; Thierry Frébourg ; François Leboulenger ; Jean-Michel DangerSource :
- Gene [ 0378-1119 ] ; 2003.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Conserved Sequence (genetics), DNA, Complementary (chemistry), DNA, Complementary (genetics), Female, Fish Proteins (genetics), Flounder (genetics), Gene Expression, Male, Membrane Proteins (genetics), Molecular Sequence Data, Ovary (metabolism), Phylogeny, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
- MESH :
- chemical , chemistry : DNA, Complementary.
- genetics : Conserved Sequence, DNA, Complementary, Fish Proteins, Flounder, Membrane Proteins.
- metabolism : Ovary.
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Female, Gene Expression, Male, Molecular Sequence Data, Phylogeny, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid.
Abstract
Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like proteins displayed a characteristic KXKXX motif which is considered as an endoplasmic reticulum targeting sequence. Gene expression, as shown by Northern blot and quantitative reverse transcription-polymerase chain reaction analysis, was significantly higher in the ovary and to a lesser extent in the brain. Xp18 transcripts were also detected by in situ hybridization in most of the circumventricular regions of the brain of adult flounders. The gene encoding the human protein is located on chromosome Xq22.1, a genome region involved in numerous genetic diseases including premature ovarian failure.
PubMed: 12706884
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000347
- to stream PubMed, to step Curation: 000347
- to stream PubMed, to step Checkpoint: 000347
- to stream Ncbi, to step Merge: 000066
- to stream Ncbi, to step Curation: 000066
- to stream Ncbi, to step Checkpoint: 000066
- to stream Main, to step Merge: 001483
- to stream Main, to step Curation: 001449
- to stream Main, to step Exploration: 001449
- to stream France, to step Extraction: 000E25
Links to Exploration step
pubmed:12706884Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary.</title><author><name sortKey="Cachot, Jerome" sort="Cachot, Jerome" uniqKey="Cachot J" first="Jérôme" last="Cachot">Jérôme Cachot</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre Cedex, France. jerome.cachot@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Bultelle, Florence" sort="Bultelle, Florence" uniqKey="Bultelle F" first="Florence" last="Bultelle">Florence Bultelle</name></author><author><name sortKey="Drouot, Laurent" sort="Drouot, Laurent" uniqKey="Drouot L" first="Laurent" last="Drouot">Laurent Drouot</name></author><author><name sortKey="Galgani, Francois" sort="Galgani, Francois" uniqKey="Galgani F" first="François" last="Galgani">François Galgani</name></author><author><name sortKey="Frebourg, Thierry" sort="Frebourg, Thierry" uniqKey="Frebourg T" first="Thierry" last="Frébourg">Thierry Frébourg</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12706884</idno><idno type="pmid">12706884</idno><idno type="wicri:Area/PubMed/Corpus">000347</idno><idno type="wicri:Area/PubMed/Curation">000347</idno><idno type="wicri:Area/PubMed/Checkpoint">000347</idno><idno type="wicri:Area/Ncbi/Merge">000066</idno><idno type="wicri:Area/Ncbi/Curation">000066</idno><idno type="wicri:Area/Ncbi/Checkpoint">000066</idno><idno type="wicri:doubleKey">0378-1119:2003:Cachot J:molecular:cloning:of</idno><idno type="wicri:Area/Main/Merge">001483</idno><idno type="wicri:Area/Main/Curation">001449</idno><idno type="wicri:Area/Main/Exploration">001449</idno><idno type="wicri:Area/France/Extraction">000E25</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary.</title><author><name sortKey="Cachot, Jerome" sort="Cachot, Jerome" uniqKey="Cachot J" first="Jérôme" last="Cachot">Jérôme Cachot</name><affiliation wicri:level="3"><nlm:affiliation>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre Cedex, France. jerome.cachot@univ-lehavre.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Ecotoxicology, UPRES-EA 3222, IFRMP 23, University of Le Havre, 25 rue Philippe Lebon, BP 540, 76058 Le Havre Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Le Havre</settlement></placeName></affiliation></author><author><name sortKey="Bultelle, Florence" sort="Bultelle, Florence" uniqKey="Bultelle F" first="Florence" last="Bultelle">Florence Bultelle</name></author><author><name sortKey="Drouot, Laurent" sort="Drouot, Laurent" uniqKey="Drouot L" first="Laurent" last="Drouot">Laurent Drouot</name></author><author><name sortKey="Galgani, Francois" sort="Galgani, Francois" uniqKey="Galgani F" first="François" last="Galgani">François Galgani</name></author><author><name sortKey="Frebourg, Thierry" sort="Frebourg, Thierry" uniqKey="Frebourg T" first="Thierry" last="Frébourg">Thierry Frébourg</name></author><author><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Conserved Sequence (genetics)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>Female</term><term>Fish Proteins (genetics)</term><term>Flounder (genetics)</term><term>Gene Expression</term><term>Male</term><term>Membrane Proteins (genetics)</term><term>Molecular Sequence Data</term><term>Ovary (metabolism)</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Conserved Sequence</term><term>DNA, Complementary</term><term>Fish Proteins</term><term>Flounder</term><term>Membrane Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ovary</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Female</term><term>Gene Expression</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Screening of a flounder ovary cDNA library with a rainbow trout p53 probe led to the isolation of a p53-unrelated cDNA encoding an unknown 161 amino acid protein. In view of its apparent molecular weight and yet unknown function, the deduced protein was named Xp18. Corresponding orthologous cDNAs or expressed sequence tags have been identified in several species including human, rodents, bovine, chicken and zebrafish and a related cDNA has also been isolated in the fruit fly. Deduced amino acid sequences appeared to be extremely well conserved throughout vertebrate evolution. Structure predictions suggested that Xp18 may correspond to an integral protein comprising four transmembrane domains. The charged C-termini of all known vertebrate Xp18-like proteins displayed a characteristic KXKXX motif which is considered as an endoplasmic reticulum targeting sequence. Gene expression, as shown by Northern blot and quantitative reverse transcription-polymerase chain reaction analysis, was significantly higher in the ovary and to a lesser extent in the brain. Xp18 transcripts were also detected by in situ hybridization in most of the circumventricular regions of the brain of adult flounders. The gene encoding the human protein is located on chromosome Xq22.1, a genome region involved in numerous genetic diseases including premature ovarian failure.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Le Havre</li></settlement></list><tree><noCountry><name sortKey="Bultelle, Florence" sort="Bultelle, Florence" uniqKey="Bultelle F" first="Florence" last="Bultelle">Florence Bultelle</name><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><name sortKey="Drouot, Laurent" sort="Drouot, Laurent" uniqKey="Drouot L" first="Laurent" last="Drouot">Laurent Drouot</name><name sortKey="Frebourg, Thierry" sort="Frebourg, Thierry" uniqKey="Frebourg T" first="Thierry" last="Frébourg">Thierry Frébourg</name><name sortKey="Galgani, Francois" sort="Galgani, Francois" uniqKey="Galgani F" first="François" last="Galgani">François Galgani</name><name sortKey="Leboulenger, Francois" sort="Leboulenger, Francois" uniqKey="Leboulenger F" first="François" last="Leboulenger">François Leboulenger</name></noCountry><country name="France"><region name="Région Normandie"><name sortKey="Cachot, Jerome" sort="Cachot, Jerome" uniqKey="Cachot J" first="Jérôme" last="Cachot">Jérôme Cachot</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/France/explor/LeHavreV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E25 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000E25 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/France
|area= LeHavreV1
|flux= France
|étape= Analysis
|type= RBID
|clé= pubmed:12706884
|texte= Molecular cloning of flounder Xp18, a newly identified highly conserved protein mainly expressed in the ovary.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i -Sk "pubmed:12706884" \
| HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a LeHavreV1
| This area was generated with Dilib version V0.6.25. |  |